Accelerated Wound Healing by Fibroblasts Differentiated from Human Embryonic Stem Cell-Derived Mesenchymal Stem Cells in a Pressure Ulcer Animal Model

Figure 4

Effective wound healing after treatment with human embryonic stem cell-derived mesenchymal stem cell-derived fibroblasts (hESC-MSC-Fbs). (a) mRNA levels of inflammatory genes were determined in the wounded dorsal skin at 4 days after three I/R cycles by the real-time polymerase chain reaction (, one-way ANOVA; , , and ). (b) An in vitro cytokine array was performed on the wounded dorsal skin at 4 days after three I/R cycles. (c) The graph shows the quantified expression levels of several cytokines that showed differences between groups (, one-way ANOVA; , , , and ). (d, e) Wounded dorsal skin tissues at 12 days after three I/R cycles were immunostained for the detection of the angiogenesis markers CD31 (d) and VEGFA (e). Tissues were counterstained with hematoxylin. The graph shows the quantified expression levels of CD-31 (d) and VEGFA (e), relatively. Image quantification was performed through an ImageJ software program. (http://www.mecourse.com/landinig/software/cdeconv/cdeconv.html).

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