Developmental Pathways Pervade Stem Cell Responses to Evolving Extracellular Matrices of 3D Bioprinted Microenvironments

Figure 2

RNA sequencing analysis of hMSCs in 3D bioprinted prisms at 0 and 28 days. (a) Hierarchical clustering of all genes with FPKM > 1 for 3D bioprinted prisms containing either BSA alone (3D/BSA), 3D/FN, 3D/Col1, or 3D/LN at day 28 and associated 2D control cultures at day 28. (b) qPCR validation of gene expression of a subset of genes. These genes were selected as they represent families of ECM proteins and differentiation markers associated with hMSC progeny. (c) PCA analysis of all genes with FPKM > 1. (d–f) Comparison of individual 3D bioprinted prisms containing ECM to bioprinted prisms with BSA only.

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