Developmental Pathways Pervade Stem Cell Responses to Evolving Extracellular Matrices of 3D Bioprinted Microenvironments
Table 1
Expression levels of ECM proteins in hMSCs cultured for 28 days in 2D standard and 3D ECM-based structure conditions.
Gene
Average FPKM
Significance
2D/D28
3D/BSA
3D/FN
3D/Col1
3D/LN
COL1A1
1694.69
1408.85
550.58
1796.03
656.82
COL1A2
1437.28
967.59
596.87
1363.82
1391.45
COL3A1
331.77
582.23
363.92
549.25
694.15
COL4A1
39.19
16.04
16.18
27.23
3.37
COL4A5
3.16
8.90
8.37
10.34
0.00
COL5A1
34.22
27.83
21.03
20.68
15.59
COL5A2
30.84
42.84
29.80
55.98
28.19
COL6A1
22.61
68.66
72.65
61.24
47.19
COL6A3
102.42
196.03
139.69
173.26
177.62
COL8A1
24.32
52.07
61.73
76.64
100.51
COL8A2
7.88
12.63
5.02
8.64
2.20
COL10A1
5.34
6.97
4.53
9.20
11.18
COL11A1
10.60
58.77
16.37
59.43
39.83
COL12A1
3.44
13.00
8.38
11.41
10.26
COL14A1
0.53
4.33
10.65
7.61
6.84
FN1
2206.39
3823.51
2129.52
4017.30
5628.25
d, h
LAMA4
15.13
36.67
30.44
30.82
46.42
LAMB1
67.78
107.45
65.75
111.18
67.06
LAMB2
11.67
4.08
7.36
8.31
14.50
LAMC1
28.32
65.12
31.50
61.57
39.43
LAMC2
7.05
11.45
9.53
15.77
0.33
CD44
96.64
120.43
132.88
179.45
168.33
ELN
16.18
2.01
0.97
1.65
0.15
FBN1
30.03
84.49
54.72
85.81
86.47
FBLN1
80.54
170.48
126.57
131.39
215.67
FBLN5
95.26
118.34
52.77
75.25
77.58
SDC2
16.83
14.14
13.80
12.82
12.50
SDC4
18.49
2.18
11.96
8.78
5.02
a, c, d, f
TNC
38.44
179.79
83.47
168.76
465.74
d, h
THBS1
299.96
696.28
239.23
538.79
819.96
THBS2
45.80
68.03
61.96
97.85
94.65
THBS3
33.11
19.98
10.57
17.22
23.76
VCAN
98.11
279.02
188.23
237.38
334.81
a, c, d, h
HAS2
234.75
307.97
100.67
401.46
505.58
Bold: reduction in expression, compared to 2D standard. Italic: increase in expression, compared to 2D standard. Underlined: FPKM < 1. a: 2D versus BSA; b: 2D versus 3D/FN; c: 2D versus 3D/Col1; d: 2D versus 3D/LN; e: BSA versus 3D/I; f: BSA versus 3D/LN; g: 3D/FN versus 3D/Col1; h: 3D/FN versus 3D/LN.
