Developmental Pathways Pervade Stem Cell Responses to Evolving Extracellular Matrices of 3D Bioprinted Microenvironments
Table 3
Expression levels of MMPS, ADAMS, and TIMPs in hMSCs cultured for 28 days in 2D standard and 3D ECM-based structure conditions.
Gene
Average FPKM
Significance
2D/D28
3D/BSA
3D/FN
3D/Col1
3D/LN
MMP1
48.12
822.48
283.69
640.14
1395.91
d
MMP2
689.96
598.60
601.51
830.16
1332.27
MMP3
8.08
10.23
3.77
8.00
19.02
MMP8
0.00
10.62
0.26
0.00
7.48
MMP11
5.08
1.71
1.71
0.00
0.00
MMP13
9.76
40.09
111.21
76.32
224.58
d, f
MMP14
2.43
3.87
1.02
1.41
0.81
MMP16
3.62
4.69
4.40
26.89
6.98
c, e, g
MMP19
14.86
12.39
7.18
29.18
10.34
MMP24
1.65
0.45
0.37
1.34
0.17
ADAM1
0.00
0.00
0.27
0.31
2.18
ADAM9
29.28
39.54
40.17
30.69
42.11
ADAM10
9.86
15.55
15.02
22.07
49.41
d, f, h
ADAM12
20.52
67.73
26.70
46.95
49.82
a, b
ADAM15
0.19
0.00
0.25
2.97
1.08
ADAM17
7.86
15.32
14.24
12.09
14.60
ADAM19
31.25
6.38
3.71
6.91
4.20
a, b, c, d
ADAM21
0.00
1.72
0.02
0.00
0.00
ADAM23
2.55
0.39
0.18
1.20
2.91
ADAM33
0.26
0.00
2.07
0.94
0.00
TIMP1
18055.04
2719.30
5834.20
2490.98
3835.62
a, c, d
TIMP2
68.19
160.04
127.25
143.54
154.66
TIMP3
1321.25
2670.46
879.00
2115.14
3145.51
h
Bold: reduction in expression, compared to 2D standard. Italic: increase in expression, compared to 2D standard. Underlined: FPKM < 1. a: 2D versus BSA; b: 2D versus 3D/FN; c: 2D versus 3D/Col1; d; 2D versus 3D/LN; e: BSA versus 3D/I; f: BSA versus 3D/LN; g: D/FN versus 3D/Col1; h: 3D/FN versus 3D/LN.
