The Effects of Hyperacute Serum on the Elements of the Human Subchondral Bone Marrow Niche

Figure 5

Culture of BMEs. Viability of BMEs cultured in serum-supplemented media (a). Serum-free medium (white bard) and medium supplemented with FCS (gray bar) or HAS (red bar). (b) Representative confocal microscopy images stained with the live/dead dye combination Calcein-AM/ethidium homodimer. Nuclei were counterstained with Hoechst. Coloured terms indicate the colour of the representative dye. Confidence interval -value 0.05 to 0.01, significant; -value 0.0001 to 0.001, extremely significant.

(a)

(b)