Conditioned Medium from Human Amnion-Derived Mesenchymal Stem Cells Regulates Activation of Primary Hepatic Stellate Cells

Figure 3

Effect of hAMSC-CM on primary HSC activation and proliferation and ECM accumulation in routine culture. (a) Immunofluorescence staining of α-SMA (green) in HSCs cultured in SM or hAMSC-CM for 48 h. Nuclei were stained by Hoechst 33342 (blue). Scale bar = 50 μm. Data are expressed as mean ± SD (n = 28 in the SM group and in the hAMSC-CM group). versus SM. (b) Expression of α-Sma in different concentrations of hAMSC-CM. Data are expressed as mean ± SD (). versus SM 100%. (c) ECM-related gene expression analysis of HSCs cultured in SM or different concentrations of hAMSC-CM. The data are expressed as mean ± SD (). and versus SM 100%. (d) Relative Mmp-13/Timp-1 expression ratio. Data are expressed as mean ± SD (). and versus SM 100%. (e) Expression of COL1 in culture media analyzed by ELISA. Data are expressed as mean ± SD (). versus SM 100%. (f) Proliferation-related gene expression analysis for Ccnb-1 and Ccnb-2. The data are expressed as mean ± SD (). versus SM. (g) Detection of proliferation of HSCs cultured in SM or hAMSC-CM by CCK-8. The data are expressed as mean ± SD ( for each time point and culture condition). versus SM.

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