Research Article
Generation of Retinal Organoids with Mature Rods and Cones from Urine-Derived Human Induced Pluripotent Stem Cells
Table 2
1st and 2nd antibodies used in this study.
(a) |
| | Primary antibody | Manufacturer | Cat. number | Dilution | Source |
| | OCT4 | Abcam | ab18976 | 1/250 | Rabbit | | NANOG | Abcam | ab21624 | 1/100 | Rabbit | | SSEA4 | Abcam | ab16287 | 1/100 | Mouse | | TRA-1-81 | Abcam | ab16289 | 1/100 | Mouse | | TRA-1-60 | Abcam | ab16288 | 1/100 | Mouse | | SOX1 | Abcam | ab87775 | 1/200 | Rabbit | | PAX6 | DSHB | 3B5 | 1/50 | Mouse | | OTX2 | Abcam | ab21990 | 1/500 | Rabbit | | LHX2 | Santa Cruz Biotechnology | sc-81311 | 1/200 | Mouse | | SIX3 | Rockland | 600-401-A26 | 1/500 | Rabbit | | MCM2 | Abcam | ab4461 | 1/400 | Rabbit | | CHX10 | Millipore | ab9016 | 1/200 | Sheep | | FIBRONECTIN | Abcam | ab2413 | 1/200 | Rabbit | | ZO-1 | Thermo Fisher | 33-9100 | 1/400 | Mouse | | BRN3 | Santa Cruz Biotechnology | sc-6026 | 1/200 | Goat | | AP2 | DSHB | 3B5a | 1/35 | Mouse | | PROX1 | Abcam | ab101851 | 1/2000 | Rabbit | | PKC-α | Abcam | ab32376 | 1/2000 | Rabbit | | CRALBP | Abcam | ab15051 | 1/500 | Mouse | | Rhodopsin | Abcam | ab3267 | 1/200 | Mouse | | S opsin | Gift from Dr. Jeremy Nathans | / | 1/5000 | Rabbit | | L/M opsin | Gift from Dr. Jeremy Nathans | / | 1/5000 | Rabbit | | Recoverin | Millipore | ab5585 | 1/500 | Rabbit | | PDE6α | Abcam | ab5665 | 1/100 | Rabbit | | Gtα-1 | Santa Cruz Biotechnology | sc-136143 | 1/2000 | Mouse |
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(b) |
| | Secondary antibodies | Manufacturer | Cat. number | Dilution |
| | Alexa Fluor 488 Donkey Anti-Goat | Invitrogen | A11055 | 1/500 | | Alexa Fluor 555 Donkey Anti-Sheep | Invitrogen | A21436 | 1/500 | | Alexa Fluor 488 Donkey Anti-Mouse | Invitrogen | A21202 | 1/500 | | Alexa Fluor 555 Donkey Anti-Mouse | Invitrogen | A31570 | 1/500 | | Alexa Fluor 488 Donkey Anti-Rabbit | Invitrogen | A21206 | 1/500 | | Alexa Fluor 555 Donkey Anti-Rabbit | Invitrogen | A31572 | 1/500 |
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