Reprogramming of Mouse Calvarial Osteoblasts into Induced Pluripotent Stem Cells
Reprogramming of osteoblasts into iPS cells. (a) Schematic illustration of iPS reprogramming strategy from sorted osteoblasts. (b) Phase (insets) and fluorescence images of osteoblasts and fibroblasts derived from transgenic mice carrying Osx1-GFP::Cre and/or Oct4-EGFP as well as their respective iPS cells at the indicated day (d) posttransduction. Scale bar: 5 μm. (c) Quantification of the number of Oct4-EGFP+ F-iPS and O-iPS colonies formed. . Data are expressed as means ± SEM. (d) Genotyping of 6 O-iPS colonies for the presence of Oct4-EGFP and Cre transgenes. Sorted GFP+ osteoblasts serve as a positive control and blank as a negative control.