Evaluation of Oxidative Stress and Mitophagy during Adipogenic Differentiation of Adipose-Derived Stem Cells Isolated from Equine Metabolic Syndrome (EMS) Horses

Figure 3

Growth kinetics and morphology of ASC_CTRL and ASC_EMS cultured under adipogenic conditions. To evaluate the proliferation rate under adipogenic differentiation, incorporation of BrdU was established (a) in both of the investigated groups. To confirm adipogenesis and establish its effectiveness, cells were stained with Oil Red O. Moreover, the secretion of MVs was evaluated using SEM imaging while the ultrastructure of cells was visualized using a TEM microscope (b). The Oil Red O-stained area was quantified using Image J (c). Using RT-PCR, expression of PPARγ (d), STAT5A (e), and SREBP 1c (f) was established. Cell cycle (DNA content) was analyzed in both of the investigated cultures using a cell analyzer (g). Obtained results indicate a more effective differentiation in control cells as they stop to proliferate in order to differentiate into adipocytes. ASC isolated from EMS individuals seems to be more resistant to adipogenic stimuli. Results are expressed as mean ± SD. Scale bar 200 μm. , , and

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