Evaluation of Oxidative Stress and Mitophagy during Adipogenic Differentiation of Adipose-Derived Stem Cells Isolated from Equine Metabolic Syndrome (EMS) Horses

Figure 8

Endoplasmic reticulum stress in ASC_CTRL and ASC_EMS cultured under adipogenic conditions after the 10th day. ER condition and distribution were visualized under fluorescent and TEM microscopes. The more intense fluorescence ER signal was noted in ASC_CTRL, where ER was robustly expanded throughout the cells (a). In the case of ASC_EMS, ER was fragmented and disintegrated as observed under TEM (b). To further investigate ER stress in investigated cells, we establish the expression of eIF2α (c), BIP (d), CHOP (e), and PERK (f). Obtained results indicated an induction of unfolded protein response and ER stress in ASC_EMS. Furthermore, we decided to evaluate ER stress in ASC niche-adipose tissue. Interestingly ER stress was also observed within adipocytes of EMS horses as increased expression of both CHOP (g) and PERK (h) transcripts was noted. Results are expressed as mean ± SD. Scale bar 250 μm. , , and

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