Evaluation of Oxidative Stress and Mitophagy during Adipogenic Differentiation of Adipose-Derived Stem Cells Isolated from Equine Metabolic Syndrome (EMS) Horses

Table 2

Sequences of primers used in qPCR.

Gene	Primer	Sequence 5–3	Amplicon length (bp)	Accession no.
PPARγ	F:	TCCCTGTTTGTGTACAGCCTT	191	XM_014846252.1
	R:	CTCCATGGCTGATTTCCCCT
STAT5A	F:	AGATGCTGGCCGAGGTCAAC	212	XM_001494738.4
	R:	AGACTTGGCCTGCTGCTCAC
SREBP-1c	F:	TCAGCGAGGCGGCTTTGGAGCAG	80	XM_008542859.1
	R:	CATGTCTTCGATGTCGGTCAG
p53	F:	TTCCGCAAGAAGGAGGAACC	114	U37120.1
	R:	TTTGGACAGAACTGCACCCT
p21	F:	GAAGAGAAACCCCCAGCTCC	241	XM_014853747.1
	R:	TGACTGCATCAAACCCCACA
BCL-2	F:	TTCTTTGAGTTCGGTGGGGT	164	XM_014843802.1
	R:	GGGCCGTACAGTTCCACAA
TET3	F:	CAGCCTGCATGGACTTCTGT	188	XM_014731089.1
	R:	GTTCTCCTCACTGCCGAACT
TET2	F:	ATCCTGATCCTGGTGTGGGA	143	XM_005607932.2
	R:	CCTTGACAGGCACAGGTTCT
DNMT1	F:	GGCGAAAGCGGACAATTCTG	90	XM_014741825.1
	R:	AGCGGTCTAGCAACTGGTTC
BECLIN-1	F:	GATGCGTTATGCCCAGATGC	233	XM_014833759.1
	R:	AACGGCAGCTCCTCTGAAAT
LC3	F:	TTACTGCTTTGCTCTGCCAC	213	XM_014835085.1
	R:	AGCTGCTTCTCCCCCTTGTA
LAMP2	F:	GCACCCCTGGGAAGTTCTTA	147	XM_014831347.1
	R:	ATCCAGCGAACACTCTTGGG
PINK	F:	GCACAATGAGCCAGGAGCTA	298	XM_014737247.1
	R:	GGGGTATTCACGCGAAGGTA
PARKIN	F:	TCCCAGTGGAGGTCGATTCT	218	XM_014858374.1
	R:	CCCTCCAGGTGTGTTCGTTT
MNF1	F:	AAGTGGCATTTTTCGGCAGG	217	XM_001495170.5
	R:	TCCATATGAAGGGCATGGGC
FIS1	F:	GGTGCGAAGCAAGTACAACG	118	XM_001504462.4
	R:	GTTGCCCACAGCCAGATAGA
EIF2α	F:	AGTCTTCAGGCATTGGCTCC	489	XM_001488848.5
	R:	CCGAGTGGGACATGTATCGG
CHOP	F:	AGCCAAAATCAGAGCCGGAA	272	XM_001488999.3
	R:	GGGGTCAAGAGTGGTGAAGG
PERK	F:	GTGACTGCAATGGACCAGGA	283	XM_014731045.1
	R:	TCACGTGCTCACGAGGATATT
BIP	F:	CTGTAGCGTATGGTGCTGCT	122	XM_005606029.2
	R:	CATGACACCTCCCACGGTTT
IL-6	F:	CGTCACTCCAGTTGCCTTCT	225	XM_014830631.1
	R:	GCCAGTACCTCCTTGCTGTT
GLUT4	F:	TGGGCTCTCTCCGTGGCCATCTT	658	NM_001081866.2
	R:	GCTGCTGGCTGAGCTGCAGCA
APLN	F:	TGGGCCTGAGATGGCTCTAT	819	XM_014729165.1
	R:	CCTCGGGGTTATTGTGGGAC
GAPDH	F:	GATGCCCCAATGTTTGTGA	250	XM_014866500.1
	R:	AAGCAGGGATGATGTTCTGG

Table 2 is reproduced from Kornicka et al. [10] (under the Creative Commons Attribution License/http://pubs.rsc.org/is/content/articlelanding/2016/ra/c6ra00029k), PPARγ—peroxisome proliferator-activated receptor gamma, STAT5A—signal transducer and activator of transcription 5A, SREBP-1c—sterol regulatory element binding transcription factor 1 (SREBF1), p53—tumor protein P53 (TP53), p21—cyclin dependent kinase inhibitor 1A (CDKN1A), BCL-2—B-cell lymphoma 2, TET3—Tet methylcytosine dioxygenase 3, TET2—Tet methylcytosine dioxygenase 2, DNMT1—DNA methyltransferase 1, BECLIN-1, LC3—microtubule-associated protein 1 light chain 3 beta (MAP1LC3B), LAMP2—lysosomal-associated membrane protein 2, PINK1—PTEN induced putative kinase 1, PARKIN—Parkin RBR E3 ubiquitin protein ligase (PRKN), MNF1—mitochondrial nucleoid factor 1, FIS1—fission, mitochondrial 1, EIF2α—eukaryotic translation initiation factor 2A, CHOP—DNA damage inducible transcript 3 (DDIT3), PERK—eukaryotic translation initiation factor 2-alpha kinase 3 (EIF2AK3), BIP—heat shock protein family A (Hsp70) member 5 (HSPA5), IL-6—interleukin 6, GLUT4—solute carrier family 2 member 4 (SLC2A4), APLN—apelin, GAPDH—glyceraldehyde 3-phosphate dehydrogenase.