In Vitro Expansion and Characterization of Mesenchymal Stromal Cells from Peritoneal Dialysis Effluent in a Human Protein Medium

Figure 1

A typical microscopic view of adherent pMSC morphology after expansion in human protein medium. Adherent cells from PD effluent of a donor were divided into two parts: one growing in FBS-containing standard medium and the other in human protein medium. After four passages (P4) in both media, unsorted cells in the plastic culture dishes displayed a typical morphology of mesenchymal cells. (a1, a2) Low-density cells; (b1, b2) high-density or confluent cells. Scale bar, 100 μm. Data were representative of five donors.