In Vitro Expansion and Characterization of Mesenchymal Stromal Cells from Peritoneal Dialysis Effluent in a Human Protein Medium

Figure 2

A different expression of CD45 or HLA-DR of pMSCs after expansion between standard medium and human protein medium. The different expression of a panel of classical MSC markers in unsorted pMSCs was compared after P4 between FBS-containing standard medium and in human protein-based xeno-free medium. Histograms of FACS analysis showed CD45 positive in pMSCs from donor #3 after expansion in the standard medium but not in the xeno-free medium (a1, a2), HLA-DR positive in pMSCs from donor #4 in the standard medium but not in the xeno-free medium (b1, b2). Dotted line: background fluorescence intensity of a control antibody, solid line: fluorescence intensity of anti-CD45 or anti-HLA-DR antibody staining.