In Vitro Expansion and Characterization of Mesenchymal Stromal Cells from Peritoneal Dialysis Effluent in a Human Protein Medium

Figure 4

A typical microscopic view of differentiated cell staining. Adherent cells from PD effluent were grown in plastic culture plates with human protein medium. After P4, unsorted pMSCs were induced to chondrocytes, adipocytes, or osteocytes by incubation with each different differentiation medium for 4 weeks. The nondifferentiated pMSCs were collected without incubation with the differentiation medium. (a1, a2) Differentiation to chondrocytes, both differentiated and nondifferentiated cells were stained with Alcian blue. Arrow: cartilage matrix in “clustering” cells. (b1, b2) Differentiation to adipocytes, both differentiated and nondifferentiated cells were stained with Oil red O. Arrows: lipid droplets. (c1, c2) Differentiation to osteocytes, both differentiated and nondifferentiated cells were stained with Alizarin red S. Red: extracellular calcium deposits. Scale bar, 100 μm. Data were presented as trilineage differentiation of representative pMSCs from ten donors.