Differential Regulation of Methylation-Regulating Enzymes by Senescent Stromal Cells Drives Colorectal Cancer Cell Response to DNA-Demethylating Epi-Drugs

Figure 3

Coculture effect on HCT116 proliferation and analysis of secretory factors in conditioned medium. (a, b) The effect of normal and senescent fibroblasts (a) and SASP of senescent fibroblasts (b) on the proliferation of HCT116. Data are mean ± SEM, , , , compared to HCT116 monocultures or 0% conditioned medium, one-sample t-test. (c) Viability of nonirradiated and irradiated HCT116 following DAC treatment. Data are mean ± SEM, , , comparing cell viability with or without conditioned medium, Student’s t-test, unpaired. (d) Increased levels of IFN-α, IL-6, IL-8, and MCP-1 in sBJ conditioned medium compared to conditioned medium from normal BJ cells and its effect on NF-κB activity. Data are mean ± SEM, , , comparing conditioned medium from BJ to sBJ, Student’s t-test, unpaired.

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