Ion Channel Expression and Characterization in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

Figure 1

Effects of Iso and CCh as well as of acidosis on APs in hiPSC-CMs. (a) Representative spontaneous APs recorded before (Ctr) and after application of 10 μM Iso and 10 μM Iso + 30 μM CCh. (b) Representative spontaneous APs recorded before (Ctr) and after application of 30 μM CCh or extracellular solution with a pH value of 6.0. (c–h) APs were recorded at a fixed frequency of 1 Hz to analyze the changes of the AP parameters induced by Iso, CCh, and acidosis. Representative traces of APs (c), mean values of resting potential (d), AP amplitude (e), maximal depolarization speed (f), APD50 (g), and APD90 (h) are shown. Values given are mean ± SEM. : number of cells. versus Ctr.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)