Ion Channel Expression and Characterization in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

Figure 4

Ion channel currents in hiPSC-CMs. Whole currents were evoked by the indicated protocols, respectively. Specific blockers or solutions were applied to confirm the identity of expected currents. (a) Representative traces of TTX- (20 μM) sensitive peak I_Na. (b) Representative traces of nifedipine- (1 μM) sensitive I_Ca-L. (c) Representative traces of mibefradil- (10 μM) sensitive I_Ca-T. (d) Representative traces of ZD7288- (30 μM) sensitive I_f. (e) Representative traces of CCh- (10 μM) activated I_KACh. (f) Representative traces of Ni²⁺- (5 mM) sensitive I_NCX. (g) Representative traces of 4-AP- (3 mM) sensitive I_to. (h) Representative traces of E-4031- (3 μM) sensitive I_Kr. (i) Representative traces of chromalol 293B- (10 μM) sensitive I_Ks. (j) Representative traces of Ba²⁺- (100 μM) sensitive I_K1. (k) Representative traces of glibenclamide- (10 μM) sensitive I_KATP. (l) Representative traces of pH- (6.0) sensitive potassium channel current. (m) Representative traces of apamin- (100 nM) sensitive I_SK1–3. (n) Representative traces of NS8593- (10 μM) sensitive I_SK1–3. (o) Representative traces of Na⁺- (50 mM) sensitive I_KNa. (p) Representative traces of capsaicipine- (100 μM) sensitive TRPV1 current. (q) Representative traces of TRAM 34- (100 nM) sensitive I_SK4. (r) Representative traces of hypotonic solution- (220 mOsm/L) activated I_Cl-vol. (s) Representative traces of niflumic acid- (100 μM) sensitive I_Cl-Ca.

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