Ion Channel Expression and Characterization in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

Table 1

Ion channel currents in hiPSC-CMs (D1).

Channel current		Ctr (pA/pF)	Blocker (pA/pF)	Sensitive current (pA/pF)	At mV	n
INa	(Days 30–40)	−30 ± 27.6	−5.9 ± 5.5	−23.7 ± 22.1	−40	7
	(Days 50–60)	−52.8 ± 44.0	−10.5 ± 3.6	−42.3 ± 35.1	−40	6
ICa-L	(Days 30–40)	−9.7 ± 1.1	−3.2 ± 0.6	−6.4 ± 1.2	10	10
	(Days 50–60)	−13.2 ± 3.8	−4.4 ± 1.2	−8.8 ± 2.5	10	10
ICa-T	(Days 30–40)	−2.1 ± 0.8	−1.0 ± 0.6	−1.1 ± 0.3	−30	7
If	(Days 30–40)	−7.5 ± 0.9	−6.4 ± 0.9	−1.2 ± 0.3	−120	8
	(Days 50–60)	−3.4 ± 0.8	−1.8 ± 0.4	−1.6 ± 0.5	−120	5
INCX	(Days 30–40)	−6.1 ± 1.0	−3.7 ± 0.8	−2.4 ± 0.6	−85	9
	(Days 50–60)	−9.6 ± 1.5	−5.5 ± 1.0	−4.2 ± 0.8	−85	15
IK1	(Days 30–40)	−13.3 ± 4.0	−12.5 ± 4.0	−0.7 ± 0.2	−120	7
	(Days 50–60)	−13.4 ± 0.9	−11.5 ± 0.9	−1.9 ± 0.4	−120	8
Ito	(Days 30–40)	13.0 ± 2.1	6.9 ± 1.5	6.1 ± 1.0	70	14
	(Days 50–60)	10.7 ± 2.1	5.7 ± 1.3	5.0 ± 0.9	70	10
IKr	(Days 30–40)	2.6 ± 0.4	1.8 ± 0.3	0.9 ± 0.2	40	8
	(Days 50–60)	2.5 ± 0.4	1.6 ± 0.3	1.0 ± 0.2	40	7
IKs	(Days 30–40)	1.7 ± 0.5	0.9 ± 0.3	0.8 ± 0.5	40	15
	(Days 50–60)	1.3 ± 0.4	0.5 ± 0.2	0.8 ± 0.3	40	10
IKATP	(Days 30–40)	−4.1 ± 0.4	−2.2 ± 0.8	−1.9 ± 0.4	−120	5
	(Days 50–60)	−4.7 ± 0.9	−2.4 ± 0.8	−2.2 ± 0.4	−120	5
IKNa	(Days 30–40)	2.2 ± 0.2	2.1 ± 0.2	0.1 ± 0.1	60	8
IK-pH	(Days 30–40)	7.0 ± 2.6	5.1 ± 2.8	1.8 ± 0.8	60	5
	(Days 50–60)	12.7 ± 3.9	9.3 ± 3.0	3.4 ± 2.1	60	4
SK1–3	(Days 30–40)	1.9 ± 0.7	1.5 ± 0.5	0.5 ± 0.4	40	10
	(Days 50–60)	3.1 ± 1.5	2.5 ± 1.2	0.6 ± 0.3	40	7
IKACh	(Days 30–40)	−6.6 ± 0.3	−4.09 ± 0.6	−2.6 ± 0.6	−120	6
	(Days 50–60)	−6.7 ± 0.3	−4.5 ± 0.3	−2.2 ± 0.3	−120	6
ITRPV1	(Days 30–40)	3.8 ± 1.4	2.4 ± 1.1	1.4 ± 0.5	40	5
	(Days 50–60)	3.5 ± 1.4	2.7 ± 1.1	0.8 ± 0.4	40	5
ICl-Ca2	(Days 30–40)	4.3 ± 1.3	3.7 ± 0.8	0.6 ± 0.8	50	6
	(Days 50–60)	4.7 ± 1.2	4.0 ± 0.9	0.7 ± 0.4	50	7
ISK4	(Days 30–40)	5.8 ± 0.8	3.0 ± 0.7	2.8 ± 0.4	50	5
	(Days 50–60)	2.6 ± 0.6	1.5 ± 0.3	1.1 ± 0.9	50	5
ICl-vol	(Days 30–40)	0.8 ± 0.2	1.1 ± 0.5	0.3 ± 0.5	60	6
	(Days 50–60)	0.9 ± 0.2	2.2 ± 0.6	1.2 ± 0.6	60	6

versus days 30–40; [Na+]i = 6 mM; [Na+]i = 50 mM; pH = 7.4; pH = 6.0; isotonic solution; hypotonic solution.