Human Umbilical Cord MSC-Derived Exosomes Suppress the Development of CCl₄-Induced Liver Injury through Antioxidant Effect

Figure 1

hucMSC-Ex suppressed CCl₄-induced mouse liver tumor growth. (a) Morphological appearance of cultured hucMSCs. Original magnification 200x. (b) Identification of hucMSC-Ex with transmission electron micrograph. Scale bar 100 nm. (c) Imaging flow cytometer analysis of phenotypic markers of hucMSC-Ex. hucMSC-Ex were positive for CD9 and CD63. (d) Western blot assay indicated the positive expression of CD9 and CD63 proteins in hucMSC-Ex. (e) Distribution of CM-DiR labeled hucMSC-Ex in CCl₄-induced hepatic carcinoma mice by in vivo fluorescent imaging. (f) Macroscopical observation of tumors in the liver surface of mice treated with PBS or hucMSC-Ex. The arrows indicated the tumors. (g) Analysis of the number and average size of tumors in the livers. Tumor size was significantly reduced in the hucMSC-Ex group (; ). (h) Representative images of H&E staining in the livers treated with PBS or hucMSC-Ex. Original magnification 100x.

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