Bidirectional Transcriptome Analysis of Rat Bone Marrow-Derived Mesenchymal Stem Cells and Activated Microglia in an In Vitro Coculture System

Figure 1

Cellular movement-related gene expression variation in rat bone marrow-derived mesenchymal stem cells (rBM-MSCs) cocultured with LPS-stimulated microglia. (a) In vitro coculture experimental design. Four different conditions were used (groups 1–4): group 1, rBM-MSCs only (control); group 2, rBM-MSCs cocultured with microglia; group 3, LPS-treated rBM-MSCs; and group 4, rBM-MSCs cocultured with LPS-stimulated microglia. (b) Gene categorisation according to subgroups related to cellular movement. Genes involved with cellular movement were categorised in subgroups (chemotaxis, homing, migration, and infiltration) based on microarrays and Ingenuity Pathway Analysis. (c) Heat map of genes related to cell migration in the 4 different groups with altered expression levels. Gene expression values are coloured from green (downregulated) to red (upregulated).

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