Bidirectional Transcriptome Analysis of Rat Bone Marrow-Derived Mesenchymal Stem Cells and Activated Microglia in an In Vitro Coculture System

Figure 5

Reduced inflammatory response in LPS-stimulated microglia cocultured with rat bone marrow-derived mesenchymal stem cells (rBM-MSCs). (a) Gene network related to inflammatory response was constructed, and cellular function was predicted algorithmically using Ingenuity Pathway Analysis. Red and green areas indicate up- and downregulated genes, respectively. Differentially expressed genes were obtained from microarray data (>1.2 fold-change). (b) Quantitative real-time PCR analysis of gene expression-related inflammation in LPS-stimulated microglia cocultured with rBM-MSCs compared to control (microglia only). (c) Activated microglia were counted in light microscopy images and quantified as the percentage of activated microglia/total cell number. Cells at the edge of the images were not counted. Scale bar: 20 μm. and versus control (microglia only).

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