Human Bone Marrow Contains Mesenchymal Stromal Stem Cells That Differentiate In Vitro into Contractile Myofibroblasts Controlling T Lymphocyte Proliferation

Figure 2

Colonization of collagen membranes by MSC. Collagen membranes were seeded with MSC from passage 1 and maintained in RPMI medium supplemented with HPL for up to 119 days, with the medium changed every 2-3 days. (a) Membranes were observed by light microscopy in the culture vessels (upper panels, scale bar is 500 μm), or after fixation and staining with Goldner’s stain (middle and lower panels, scale bars are 500 and 100 μm, resp.). Upper left panel: empty membrane; upper right panel: MSC-loaded membrane after 9 days of culture. Middle left panel: empty membrane section; middle right panel: MSC-laden membrane section cultured for 19 days. Lower panels: high magnification of a MSC-laden membrane section either in its center (left), or in the periphery (right). (b) Approximate membrane surface expressed in mm² in function of culture time. Data of (a) are of one experiment representative of 5 and data of (b) are the mean ± SD of 19 experiments until day 21 of culture, and of at least 3 experiments thereafter. Original magnifications of (a): higher panels 10x, middle panels 5x, and lower panels 20x.

(a)

(b)