Guiding the Differentiation Direction of Pancreatic Islet-Derived Stem Cells by Glycated Collagen

Figure 3

Adipogenic differentiation of PI-MSCs on modified collagen. The effect of the glycated collagens on differentiation was compared with cells differentiated on the uncoated glass surface (a). The stem cells were seeded on the glass surface coated with unmodified Col (b), Col-G (c), Col-M (d), Col-A (e), and Col-R (f) and differentiated for 22 days. The adipogenic differentiation was assessed by immunohistochemical staining for adiponectin (green; a–f) and Western blot analysis for adiponectin (g). The nuclei were stained with DAPI (blue). Scale bar: 100 μm.

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