Guiding the Differentiation Direction of Pancreatic Islet-Derived Stem Cells by Glycated Collagen

Figure 4

Osteogenic differentiation of PI-MSCs on modified collagen. The stem cells were seeded on the glass surface coated with Col (b), Col-G (c), Col-M (d), Col-A (e), and Col-R (f) and differentiated for 24 days. The calcium deposits (red) was analyzed by Alizarin Red S staining (a–f), and the differentiation was confirmed by Western blot analysis for osteocalcin (g). The effect of glycated collagens on differentiation was determined by comparing with the cells differentiated on the uncoated glass surface (a). Scale bar: 100 μm.

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