Ceramic Hollow Fibre Constructs for Continuous Perfusion and Cell Harvest from 3D Hematopoietic Organoids

Figure 1

Schematics for hollow fibre cell filtration experiments. (a) Cross-flow filtration: cells are inoculated within the abluminal, shell region, filtered through the fibre into the perfused lumen and are collected at the fibre effluent. (b) Dead-end filtration: a solution of cells is perfused into fibre lumens, which are blocked at the exit with a resin solution so cells must pass through the fibre into the shell and are collected at an opening in the shell. (c) HFBR culture: cells are inoculated into a 3D porous scaffold formed around the fibre, which is perfused for 21 days. Red, blue, and black arrows indicate flow direction, cell movement, and commented regions.

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