Ceramic Hollow Fibre Constructs for Continuous Perfusion and Cell Harvest from 3D Hematopoietic Organoids

Figure 4

Cross-flow filtration efficiency. (a) Number of viable and (b) percent enucleate cells collected at fibre outlets counted by trypan blue and methylene blue dye exclusion at different time points of filtration. (c) Comparison of cell viability within the water (left) and DMSO (right) fibres after 24 hours of perfusion using confocal microscopy by detection of calcein AM (green), ethidium homodimer-1 (red), and laser reflectance (grey) (100 μm scale). (d) Comparison of cells types remaining within fibres by detection of (top) nuclei (DAPI; blue), red blood cell marker CD235a (green), platelet marker CD61 (yellow), plasma membranes (CellMask; red), and laser reflectance (grey) and (below) single stains of hatched box regions (100 μm scale).

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