Ceramic Hollow Fibre Constructs for Continuous Perfusion and Cell Harvest from 3D Hematopoietic Organoids

Figure 6

Long-term 3D perfusion hollow fibre bioreactor culture. (a) Measurements of glucose (top), lactate (middle), and pH (bottom) measured at HFBR influent, effluent, and static supernatant every 2 days during culture. (b) Comparison of cells per mL of culture volume extracted at day 21 within perfused HFBR versus unperfused, static scaffold cultures. (c) Confocal microscope image of HFBR cross section at day 21 detecting CD235a (pink), EPO-R (yellow, indicated by yellow arrowheads), nuclei (DAPI; blue), and laser reflection (grey) (100 μm scale bar). SEM images of HFBR cross sections at day 21 of culture highlighting diverse cell morphologies near HFs (d, 200 μm scale bar) or further into the bulk HFBR scaffold (e, 100 μm scale bar).

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