Research Article

Ceramic Hollow Fibre Constructs for Continuous Perfusion and Cell Harvest from 3D Hematopoietic Organoids

Table 1

Primary antihuman antibody stains implemented for flow cytometry and confocal microscopy. Including primary antibody host species, conjugation if applicable, a brief description, final stained concentration or dilution, clone (if monoclonal) or AbCam product number (if polyclonal), and whether used in flow cytometry (flow) or confocal microscopy (confocal).

AntibodyDescriptionConcentrationCloneApplication

Mouse CD235a-PEMature red cell12.5 μL/mLGA-R2Flow
Mouse CD61-APCPlatelet42 μL/mLVI-PL2Flow
Mouse IgG2b-PECD235a Iso12.5 μL/mL27-35Flow
Mouse IgG1-APCCD61 Iso42 μL/mLMOPC-21Flow
Mouse CD235a-PEMature red cell2.5 μg/mLGA-R2Confocal
Rat CD235aMature red cell1.7 μg/mLYTH89.1Confocal
Mouse EPO-RErythroblast33 μg/mLMM-0031-6G7Confocal
Mouse CD36-APCErythroblast42 μL/mLCB38Confocal
Rabbit CD61Platelet8.4 μg/mLEPR2417YConfocal
Rabbit CD71Late erythroblast8.3 μg/mLPoly; ab84036Confocal
Rabbit OSxPreosteoblast8.3 μg/mLPoly; ab94744Confocal
Mouse OPNOsteoblast3.3 μg/mL53Confocal
Mouse CD31Endothelial33 μL/mLJC/70AConfocal
Rat IgG2bCD235a Iso1.7 μg/mLRTK4530Confocal
Rabbit IgGCD61/CD71 Iso8.3 μg/mLEPR25AConfocal
Mouse IgGOPN/CD31 Iso33 μg/mLPoly; ab37355Confocal
Mouse IgG2b-PECD235a Iso2.5 μg/mL27-35Confocal
Mouse IgM-APCCD61 Iso42 μL/mLG155-228Confocal

PE: phycoerythrin; APC: allophycocyanin; EPO-R: EPO-receptor; OSx: osterix; OPN: osteopontin; Iso: isotype; Poly: polyclonal.