Ceramic Hollow Fibre Constructs for Continuous Perfusion and Cell Harvest from 3D Hematopoietic Organoids

Table 2

Filtrate collected within 6 hours of cross-flow and dead-end filtration for water and DMSO fibre types. Filtration rate, viability, and enucleated fraction were assessed by manual cell counting on a hemocytometer using trypan blue or methylene blue dye exclusion. Reticulocyte purity was measured by flow cytometry and corresponded to a calcein AM-positive, Hoechst-negative, and CD235a-positive fraction.

Platform	Cross-filtration		Dead-end filtration
Fibre	Water	DMSO	Water	DMSO
Filtration rate (cells/h)	7 × 10^5	44 × 10^5	1.6 × 10^8	1.8 × 10^8
per fibre surface area (cells/h × cm^2)	6 × 10^4	45 × 10^4	24 × 10^7	27 × 10^7
Cell viability (%)	88	89	96	99
Enucleate purity (%)	92	81	84	81
Reticulocyte purity (%)	1	9	74	71