High Throughput Screening of Additives Using Factorial Design to Promote Survival of Stored Cultured Epithelial Sheets

Table 3

Effect of different additives on the number of live cells after one-week storage.

Additives	F value	p value prob > F	Effect
Adenosine (5 mM)	42.31	<0.0001	−
L-Ascorbic acid (50 μg/ml)	0.20	0.6575
Glycerol (1%)	16.19	0.0002	+
Antimycin A (20 nM)	12.09	0.0010	−
L-Ascorbic acid and Glycerol (50 ug/ml and 1%, respectively)	10.25	0.0022	+

F value: test for comparing model variance with residual variance; p value prob > F: probability of observed F value if the null hypothesis is true (small value call for rejection of the null hypothesis). Only additives involved in a significant or positive effect on viability are shown. Parentheses indicate tested concentrations of the respective additives. All additives were added to MEM basic storage medium.