High Throughput Screening of Additives Using Factorial Design to Promote Survival of Stored Cultured Epithelial Sheets

Table 6

Morphology evaluations using the reduced quadratic mixture model.

Additives	F value	p value prob > F	Effect
Carnosine (40 mM)	9.31	0.0037	+
Fenoldopam mesylate (10 μM)	48.90	<0.0001	+
Carnosine + DMSO (0.2%)	8.74	0.0048	−
Carnosine + fenoldopam mesylate	30.96	<0.0001	+
Carnosine + glycerol (6%)	9.20	0.0039	−
Carnosine + LIF (40 ng/ml)	8.19	0.0062	+
DMSO + fenoldopam mesylate	69.17	<0.0001	−
Fenoldopam mesylate + glycerol	64.85	<0.0001	+
Fenoldopam mesylate + LIF	25.24	<0.0001	−

F value: test for comparing model variance with residual variance; p value prob > F: probability of observed F value if the null hypothesis is true (small value call for rejection of the null hypothesis) Parentheses indicate maximal tested concentrations of the relevant additives and not necessarily the optimal levels.