Isolation and Characterisation of Mesenchymal Stem Cells from Rat Bone Marrow and the Endosteal Niche: A Comparative Study

Figure 2

Analysis of MSC characteristics for cells isolated from compact bone and bone marrow stroma. (a) Cumulative population doubling (PDs) of CB-MSC and BM-MSC isolates, performed on two separate occasions, demonstrated high ability for self-replication after PD10, approximately 60 days in culture. (b) Both CB-MSCs and BM-MSCs expressed classical MSC markers, in addition to certain embryonic markers. Osteogenic marker, Runx2, was strongly expressed by both cell populations at all PDs examined, but were negative for chondrogenic marker, Sox9, and adipogenic marker, PPARγ. CB-MSCs demonstrated the additional presence of Oct4 and CD34. (c) Colony-forming efficiency (CFEs) demonstrated lower efficiency for CB-MSC at PD15 compared to BM-MSC at PD15 and PD50. Statistically significant differences between respective populations are shown. and .

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