Isolation and Characterisation of Mesenchymal Stem Cells from Rat Bone Marrow and the Endosteal Niche: A Comparative Study

<div>Adipogenic induction of CB-MSCs and BM-MSCs at PD50. (a) CB-MSCs and (b) BM-MSCs were cultured in adipogenic medium for 14 days and stained with Oil Red O to detect neutral lipid droplet formation within the cultured cells. Potency was greater for BM-MSCs with results correlating with (c) gene expression of adipogenic markers in MSCs at PD50 quantified by qPCR. Values were normalised to the expression of <i>β</i>-actin, and expression following 14 days in adipogenic media is presented as a fold increase or decrease compared to levels in cells cultured in basal media. 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Stem Cells International

fig6

Figure 6

Figure 6: Isolation and Characterisation of Mesenchymal Stem Cells from Rat Bone Marrow and the Endosteal Niche: A Comparative Study 