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Stem Cells International
Volume 2018 (2018), Article ID 6869128, 14 pages
https://doi.org/10.1155/2018/6869128
Research Article

Isolation and Characterisation of Mesenchymal Stem Cells from Rat Bone Marrow and the Endosteal Niche: A Comparative Study

1Oral and Biomedical Sciences, School of Dentistry and Cardiff Institute Tissue Engineering and Repair, Cardiff University, Cardiff, UK
2School of Dental Sciences, University Sains Malaysia, Kelantan, Malaysia

Correspondence should be addressed to Rachel J. Waddington; ku.ca.ffidrac@jrnotgniddaw

Received 4 August 2017; Revised 20 October 2017; Accepted 5 November 2017; Published 22 March 2018

Academic Editor: Kequan Guo

Copyright © 2018 Norhayati Yusop et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. S. P. Bruder, N. Jaiswal, and S. E. Haynesworth, “Growth kinetics, self-renewal, and the osteogenic potential of purified human mesenchymal stem cells during extensive subcultivation and following cryopreservation,” Journal of Cellular Biochemistry, vol. 64, no. 2, pp. 278–294, 1997. View at Publisher · View at Google Scholar
  2. M. F. Pittenger, A. M. Mackay, S. C. Beck et al., “Multilineage potential of adult human mesenchymal stem cells,” Science, vol. 284, no. 5411, pp. 143–147, 1999. View at Publisher · View at Google Scholar · View at Scopus
  3. T. Squillaro, G. Peluso, and U. Galderisi, “Clinical trials with mesenchymal stem cells: an update,” Cell Transplantation, vol. 25, no. 5, pp. 829–848, 2016. View at Publisher · View at Google Scholar · View at Scopus
  4. P. H. Krebsbach, S. A. Kuznetsov, P. Bianco, and P. G. Robey, “Bone marrow stromal cells: characterization and clinical application,” Critical Reviews in Oral Biology & Medicine, vol. 10, no. 2, pp. 165–181, 1999. View at Publisher · View at Google Scholar · View at Scopus
  5. S. Chatterjee, R. K. Dutta, P. Basak et al., “Alteration in marrow stromal microenvironment and apoptosis mechanisms involved in aplastic anemia: an animal model to study the possible disease pathology,” Stem cells International, vol. 2010, Article ID 932354, 12 pages, 2010. View at Publisher · View at Google Scholar · View at Scopus
  6. P. Fiorina, G. Pietramaggiori, S. S. Scherer et al., “The mobilization and effect of endogenous bone marrow progenitor cells in diabetic wound healing,” Cell Transplantation, vol. 19, no. 11, pp. 1369–1381, 2010. View at Publisher · View at Google Scholar · View at Scopus
  7. C. Scotti, B. Tonnarelli, A. Papadimitropoulos et al., “Recapitulation of endochondral bone formation using human adult mesenchymal stem cells as a paradigm for developmental engineering,” Proceedings of the National Academy of Sciences, vol. 107, no. 16, pp. 7251–7256, 2010. View at Publisher · View at Google Scholar · View at Scopus
  8. L. B. To, J. P. Levesque, and K. E. Herbert, “How I treat patients who mobilize hematopoietic stem cells poorly,” Blood, vol. 118, no. 17, pp. 4530–4540, 2011. View at Publisher · View at Google Scholar · View at Scopus
  9. Q. Chen, P. Shou, C. Zheng et al., “Fate decision of mesenchymal stem cells: adipocytes or osteoblasts?” Cell Death & Differentiation, vol. 23, no. 7, pp. 1128–1139, 2016. View at Publisher · View at Google Scholar · View at Scopus
  10. K. C. Russell, D. G. Phinney, M. R. Lacey, B. L. Barrilleaux, K. E. Meyertholen, and K. C. O'Connor, “In vitro high-capacity assay to quantify the clonal heterogeneity in trilineage potential of mesenchymal stem cells reveals a complex hierarchy of lineage commitment,” Stem Cells, vol. 28, no. 4, pp. 788–798, 2010. View at Publisher · View at Google Scholar · View at Scopus
  11. S. James, J. Fox, F. Afsari et al., “Multiparameter analysis of human bone marrow stromal cells identifies distinct immunomodulatory and differentiation-competent subtypes,” Stem Cell Reports, vol. 4, no. 6, pp. 1004–1015, 2015. View at Publisher · View at Google Scholar · View at Scopus
  12. E. B. Anokhina and L. B. Buravkova, “Heterogenecity of stromal cell precursers isolated from rat bone marrow,” Cell and Tissue Biology, vol. 1, no. 1, pp. 1–7, 2007. View at Publisher · View at Google Scholar · View at Scopus
  13. M. Dominici, K. Le Blanc, I. Mueller et al., “Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement,” Cytotherapy, vol. 8, no. 4, pp. 315–317, 2006. View at Publisher · View at Google Scholar · View at Scopus
  14. D. G. Phinney, G. Kopen, R. L. Isaacson, and D. J. Prockop, “Plastic adherent stromal cells from the bone marrow of commonly used strains of inbred mice: variations in yield, growth, and differentiation,” Journal of Cellular Biochemistry, vol. 72, no. 4, pp. 570–585, 1999. View at Publisher · View at Google Scholar
  15. B. Neuhuber, S. A. Swanger, L. Howard, A. Mackay, and I. Fischer, “Effects of plating density and culture time on bone marrow stromal cell characteristics,” Experimental Hematology, vol. 36, no. 9, pp. 1176–1185, 2008. View at Publisher · View at Google Scholar · View at Scopus
  16. P. H. Jones and F. M. Watt, “Separation of human epidermal stem cells from transit amplifying cells on the basis of differences in integrin function and expression,” Cell, vol. 73, no. 4, pp. 713–724, 1993. View at Publisher · View at Google Scholar · View at Scopus
  17. G. P. Dowthwaite, J. C. Bishop, S. N. Redman et al., “The surface of articular cartilage contains a progenitor cell population,” Journal of Cell Science, vol. 117, no. 6, pp. 889–897, 2004. View at Publisher · View at Google Scholar · View at Scopus
  18. R. J. Waddington, S. J. Youde, C. P. Lee, and A. J. Sloan, “Isolation of distinct progenitor stem cell populations from dental pulp,” Cells Tissues and Organs, vol. 189, no. 1–4, pp. 268–274, 2009. View at Publisher · View at Google Scholar · View at Scopus
  19. P. Pimton, S. Sarkar, N. Sheth et al., “Fibronectin-mediated upregulation of α5β1 integrin and cell adhesion during differentiation of mouse embryonic stem cells,” Cell Adhesion & Migration, vol. 5, no. 1, pp. 73–82, 2011. View at Publisher · View at Google Scholar · View at Scopus
  20. J. Harrington, A. J. Sloan, and R. J. Waddington, “Quantification of clonal heterogeneity of mesenchymal progenitor cells in dental pulp and bone marrow,” Connective Tissue Research, vol. 55, Supplement 1, pp. 62–67, 2014. View at Publisher · View at Google Scholar · View at Scopus
  21. J. H. Sung, H. M. Yang, J. B. Park et al., “Isolation and characterization of mouse mesenchymal stem cells,” Transplantation Proceedings, vol. 40, no. 8, pp. 2649–2654, 2008. View at Publisher · View at Google Scholar · View at Scopus
  22. Y. Nakamura, F. Arai, H. Iwasaki et al., “Isolation and characterization of endosteal niche cell populations that regulate hematopoietic stem cells,” Blood, vol. 116, no. 9, pp. 1422–1432, 2010. View at Publisher · View at Google Scholar · View at Scopus
  23. H. Zhu, Z. K. Guo, X. X. Jiang et al., “A protocol for isolation and culture of mesenchymal stem cells from mouse compact bone,” Nature Protocols, vol. 5, no. 3, pp. 550–560, 2010. View at Publisher · View at Google Scholar · View at Scopus
  24. E. B. Pierantozzi Gava, I. Manini, F. Roviello, G. Marotta, M. Chiavarelli, and V. Sorrentino, “Pluripotency regulators in human mesenchymal stem cells: expression of NANOG but not of OCT-4 and SOX-2,” Stem Cells and Development, vol. 20, no. 5, pp. 915–923, 2011. View at Publisher · View at Google Scholar · View at Scopus
  25. T. M. Liu, N. Y. N. Wu, X. M. Guo, and J. H. P. Hui, “Effects of ectopic Nanog and Oct4 overexpression on mesenchymal stem cells,” Stem Cells and Development, vol. 18, no. 7, pp. 1013–1022, 2009. View at Publisher · View at Google Scholar · View at Scopus
  26. C.-S. Lin, H. Ning, G. Lin, and T. F. Lue, “Is CD34 truly a negative marker for mesenchymal stromal cells?” Cytotherapy, vol. 14, no. 10, pp. 1159–1163, 2012. View at Publisher · View at Google Scholar · View at Scopus
  27. Y. Kunisaki, I. Bruns, C. Scheiermann, J. Ahmed, and S. Pinho, “Arteriolar niches maintain haematopoietic stem cell quiescence,” Nature, vol. 502, no. 7473, pp. 637–643, 2013. View at Publisher · View at Google Scholar · View at Scopus
  28. L. E. Silberstein, “A new image of the hematopoietic stem cell vascular niche,” Cell Stem Cell, vol. 13, no. 5, pp. 514–516, 2013. View at Publisher · View at Google Scholar
  29. S. J. Greco, K. Liu, and P. Rameshwar, “Functional similarities among genes regulated by Oct4 in human mesenchymal and embryonic stem cells,” Stem Cells, vol. 25, no. 12, pp. 3143–3154, 2007. View at Publisher · View at Google Scholar · View at Scopus
  30. X. Meng, R. J. Su, D. J. Baylink et al., “Rapid and efficient reprogramming of human fetal and adult blood CD34+ cells into mesenchymal stem cells with a single factor,” Cell Research, vol. 23, no. 5, pp. 658–672, 2013. View at Publisher · View at Google Scholar · View at Scopus
  31. T. Komori, “Regulation of osteoblast differentiation by transcription factors,” Journal of Cellular Biochemistry, vol. 99, no. 5, pp. 1233–1239, 2006. View at Publisher · View at Google Scholar · View at Scopus
  32. S. Gronthos, A. C. Zannettino, S. J. Hay et al., “Molecular and cellular characterisation of highly purified stromal stem cells derived from human bone marrow,” Journal of Cell Science, vol. 116, no. 9, pp. 1827–1835, 2003. View at Publisher · View at Google Scholar · View at Scopus
  33. S. W. Kim, S. J. Hera, S. Y. Kima, and C. S. Shina, “Ectopic overexpression of adipogenic transcription factors induces transdifferentiation of MC3T3-E1 osteoblasts,” Biochemical and Biophysical Research Communications, vol. 327, no. 3, pp. 811–819, 2005. View at Publisher · View at Google Scholar · View at Scopus
  34. K. F. Macleod, N. Sherry, G. Hannon et al., “p53-dependent and independent expression of p21 during cell growth, differentiation, and DNA damage,” Genes and Development, vol. 9, no. 8, pp. 935–944, 1995. View at Publisher · View at Google Scholar · View at Scopus
  35. Q. Huang, M. Liu, X. Du et al., “Role of p53 in preadipocyte differentiation,” Cell Biology International, vol. 38, no. 12, pp. 1384–1393, 2014. View at Publisher · View at Google Scholar · View at Scopus
  36. T. Velletri, N. Xie, Y. Wang et al., “P53 functional abnormality in mesenchymal stem cells promotes osteosarcoma development,” Cell Death Disease, vol. 7, no. 1, article e2015, 2015. View at Publisher · View at Google Scholar · View at Scopus
  37. A. V. Molofsky, R. Pardal, T. Iwashita, I. K. Park, M. F. Clarke, and S. J. Morrison, “Bmi-1 dependence distinguishes neural stem cell self-renewal from progenitor proliferation,” Nature, vol. 425, no. 6961, pp. 962–967, 2003. View at Publisher · View at Google Scholar · View at Scopus
  38. I. K. Park, D. Qian, M. Kiel et al., “Bmi-1 is required for maintenance of adult self-renewing haematopoietic stem cells,” Nature, vol. 423, no. 6937, pp. 302–305, 2003. View at Publisher · View at Google Scholar · View at Scopus
  39. J. Campisi, F. d'Adda, and F. di Fagagna, “Cellular senescence: when bad things happen to good cells,” Nature Reviews Molecular Cellular Biology, vol. 8, no. 9, pp. 729–740, 2007. View at Publisher · View at Google Scholar · View at Scopus
  40. S. N. Datta and A. B. Abou-Samra, “PTH and PTHrP signaling in osteoblasts,” Cellular Signalling, vol. 21, no. 8, pp. 1245–1254, 2009. View at Publisher · View at Google Scholar · View at Scopus
  41. L. Song and R. S. Tuan, “Transdifferentiation potential of human mesenchymal stem cells derived from bone marrow,” FASEB Journal, vol. 18, no. 9, pp. 980–982, 2004. View at Publisher · View at Google Scholar · View at Scopus
  42. O. Tecles, P. Laurent, S. Zygouritsas, A. S. Burger, J. Camps, and J. Dejou, “Activation of human dental pulp progenitor/stem cells in response to odontoblast injury,” Archives of Oral Biology, vol. 50, no. 2, pp. 103–108, 2005. View at Publisher · View at Google Scholar · View at Scopus