Research Article

Isolation and Characterisation of Mesenchymal Stem Cells from Rat Bone Marrow and the Endosteal Niche: A Comparative Study

Table 1

Primer sequences for RT-PCR and qPCR analysis.

GenePrimer sequenceLength (bp)Application

CD73F: 5 TCCCGCGGCTGCTACGGCACCCAAGTG-3
R: 5-ACCTTGGTGAAGAGCCGGGCCACGCCG-3
204RT-PCR
CD90F: 5-CCTGACCCGAGAGAAGAA-3
R: 5-TGAAGTTGGCTAGAGTAAGGA-3
125RT-PCR
CD105F: 5-ACATGGTGCCCACACCCGCAGCTGGCA-3
R: 5-CACTGCCACCACGGGCTCCCGCTTGCT-3
263RT-PCR
CD45F: 5-AGCAATACCAGTTCCTCTATGA-3
R: 5-TCCGTCCACTTCGTTATGA-3
113RT-PCR
CD34F: 5-GTCACACTGCCTACTACTTC-3
R: 5-TCCTCGGATTCCTGAACAT-3
210RT-PCR
NanogF: 5-GGGGATTCCTCGCCGATGCCTGCCGTT-3
R: 5-GGGATACTCCACCGGCGCTGAGCCCTT-3
477RT-PCR
Oct4F: 5-GCCCACCTTCCCCA TGGCTGGACACCT-3
R: 5-GCAGGGCCTCGAAGCGGCAGA TGGTTG-3
563RT-PCR
Runx2F: 5-CCAGATGGGACTGTGGTTACC-3
R: 5-ACTTGGTGCAGAGTTCAGGG-3
381RT-PCR
OCNF: 5-ACAGACAAGTCCCACACAGCAACT-3
R: 5-CCTGCTTGGACA TGAAGGCTTTGT-3
161RT-PCR
PPARγF: 5-GGAAAGACAACAGACAAA TCAC-3
R: 5-GAACTTCACAGCAAACTCAAAC-3
408RT-PCR
Sox9F: 5-CCCTTCAACCTCCCACACTACAGC-3
R: 5-TGTGTAGACGGGTTGTTCCCAGTG-3
249RT-PCR
rTERTF: 5-GACATGGAGAACAAGCTGTTTGC-3
R: 5-ACAGGGAAGTTCACCACTGTC-3
185RT-PCR
p53F: 5-ACAGCGTGGTGGTACCGTAT-3
R: 5-GGAGCTGTTGCACATGTACT-3
83RT-PCR
p21waf1F: 5-TCTTGCACTCTGGTGTCTCA-3
R: 5-GGGCTTTCTCTTGCAGAA-3
147RT-PCR
p16INK4AF: 5-TGCAGATAGACTAGCCAGGGC-3
R: 5-CTCGCAGTTCGAATCTGCAC-3
184RT-PCR
β-ActinF: 5-TGAAGATCAAGATCATTGCTCCTCC-3
R: 5-CTAGAAGCATTTGCGGTGGACGATG-3
155RT-PCR
OSXF: 5-GCTTTTCTGTGGCAAGAGGTTC-3
R: 5-CTGATGTTTGCTCAAGTGGTCG-3
200qPCR
OCNF: 5-ACAGACAAGTCCCACACAGCAACT-3
R: 5-CCTGCTTGGACATGAAGGCTTTGT-3
161qPCR
FABP4F: 5-GGAATTCGATGAAATCACCCC-3
R: 5-TGGTCGACTTTCCATCCCACT-3
104qPCR
C/EBPαF: 5-GGGAGAACTCTAACTCCCCCAT-3
R: 5-CTCTGGAGGTGGCTGCTCATC-3
82qPCR
LPLF: 5-AGGTCAGAGCCAAGAGAAGCA-3
R: 5-GGAGTAGGTTTTATTTGTGGCG-3
215qPCR
β-ActinF: 5-GGGTCGAGTCCGCGTCCAC-3
R: 5-CGACGAGCGCAGCGATATC-3
108qPCR