TGFβ1-Induced Differentiation of Human Bone Marrow-Derived MSCs Is Mediated by Changes to the Actin Cytoskeleton
TGFβ1 does not affect hMSC proliferation or viability. (a) Chart showing the relative hMSC viability in the absence (CNT) or presence (TGFB1) of TGFβ1, as determined by alamarBlue assay reagent. Shown are cell viabilities on days 2 (D2), 3 (D3), and 4 (D4) of culture. (b) Real-time proliferation assay data using the xCELLigence RTCA DP system for hMSC cells with and without TGFβ1 treatment. Lower panel: cell proliferation was measured at 15-minute intervals for a total duration of 24 hours. Upper panel: summary data showing cellular proliferation after 24 hours. Data are shown as the mean ± SD of two independent experiments (). NS: not significant.
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