Bcl-2 Overexpression Improves Survival and Efficacy of Neural Stem Cell-Mediated Enzyme Prodrug Therapy

Figure 2

Bioactive effects of Bcl-2 expression in NSCs. (a) Representative fluorescent images of NSC cultures after a 96 hr incubation in increasing H₂O₂ doses. Live and dead cells were, respectively, visualized with calcein-AM (green) and ethidium bromide (red). (b) Absolute ATP content present in culture was quantified and normalized with respect to values observed when parental NSCs were cultured with no exogenous H₂O_2. (c) Tumor tropism of parental and transduced/transfected NSCs in response to U87 glioma-conditioned media measured in vitro. Data are expressed as percentage of migrated cells, where 100% is 1 × 10⁵. (d) Representative parental and transduced/transfected NSC cultures immunostained for lineage-specific protein. (e) Flow cytometry analysis of E. coli cytosine deaminase expression parental and transduced/transfected NSCS immunostained with anti-CD.

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