Low Magnitude of Compression Enhances Biosynthesis of Mesenchymal Stem Cells towards Nucleus Pulposus Cells via the TRPV4-Dependent Pathway

Table 1

Real-time polymerase chain reaction primers.

Gene	Sequence		Size
Aggrecan	Forward	5 ATTTCCACACGCTACACCCTG 3	164 bp
	Reverse	5 TGGATGGGGTATCTGACTGTC 3
Type II	Forward	5 CAGGATGCCCGAAAATTAGGG 3	132 bp
Collagen α1	Reverse	5 ACCACGATCACCTCTGGGT 3
Sox-9	Forward	5 TCAACGGCTCCAGCAAGAACAAG 3	194 bp
	Reverse	5 CTCCGCCTCCTCCACGAAGG 3
GAPDH	Forward	5 GGAGTTGCTGTTGAAGTCGCA 3	532 bp
	Reverse	5 GGAGTTGCTGTTGAAGTCGCA 3

Primers for aggrecan, type II collagen α1, Sox-9, and GAPDH were designed from Mus musculus gene sequences obtained from the NCBI GenBank and RefSeq databases using Primer 5.0 software.