Laminin 521 Stabilizes the Pluripotency Expression Pattern of Human Embryonic Stem Cells Initially Derived on Feeder Cells

Figure 1

hES cells derived on hFFs and cultured to p4 on LN521. All cell lines (HS360, HS364, HS380, HS401, and HS420) cultured on LN521 exhibited typical hES cell morphology at p4 (a). The hierarchical clusters of gene expression levels for all the cell lines analysed by TLD arrays show a heterogeneous expression profile specific for every cell line. Red colour represents high gene expression (low dCT value) and green colour represents low gene expression (high dCT value) (b). As shown by the Venn diagram, all five cell lines exhibited higher expression of nine stemness-related genes and lower expression of four genes related to differentiation (c).

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(b)

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