Laminin 521 Stabilizes the Pluripotency Expression Pattern of Human Embryonic Stem Cells Initially Derived on Feeder Cells

Figure 3

Homogenization of the expression of pluripotency genes and genes related to male germ cells and somatic gonadal cells after prolonged culture of hES cells on LN521 presented as heat maps for dCT values on hFFs at p4 and LN521 at p4 and p9. Red colour represents high gene expression (low dCT value) and green colour represents low gene expression (high dCT value); undetected (low/no expression) samples was given a dCT value of 15. (Below) a table of the mean and SD values of the five cell lines showing the relative expression values of pluripotency genes and genes related to stemness and male gonadal cells to GAPDH. The coefficient of variation is calculated as SD/mean and the variations among the lines are classified into four groups: less than 10% (green), 11% to 25% (yellow), 26%–50% (orange), and more than 50% (red).