D-Mannose Enhanced Immunomodulation of Periodontal Ligament Stem Cells via Inhibiting IL-6 Secretion

Figure 2

M-hPDLSCs induced more T cell differentiation into Tregs. (a, b) The results of ELISA showed that there was no difference in PGE2 and TGFβ1 between T + M-hPDLSCs and T + G-hPDLSCs. (c, d) RT-PCR results showed that there is no difference in Nos2 and Ido1 between T + M-hPDLSCs and T + G-hPDLSCs. (e, f) FoxP3 have been detected by flow cytometry. Compared with T + G-hPDLSCs, more FoxP3 was detected in T + M-hPDLSCs. (g, h) IFNγ and IL-17 have been detected by flow cytometry. More IFNγ could be detected in T + M-hPDLSCs. There was no difference in IL-17 between T + G-hPDLSCs and T + M-hPDLSCs. (j, k) There was no difference in IL-4 between T + G-hPDLSCs and T + M-hPDLSCs. Student’s t-test was used to analyze statistical significance. All error bars represent s.d. (). and .

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