(a) Canine purified adipose MSCs at 20x magnification. (b) Differentiation assay chondrogenesis stained by Alcian blue. (c) Osteogenesis, alkaline phosphatase-positive colonies stained with Alizarin Red. (d) FACS analysis of the cultured canine adipose MSCs. Two positive and two negative CD markers for MSCs were used to identify the cells. MSCs were positive for CD90 and CD44 and negative for CD34 and CD45 cell markers.