Research Article

Induction of Expression of CD271 and CD34 in Mesenchymal Stromal Cells Cultured as Spheroids

Figure 3

ATMSCs cultured in DMEM/10 or in PCM in 2D were induced to differentiate into adipocytes or osteoblasts. Lipid-containing vacuoles of adipocytes were stained with Oil Red O and the calcium-rich extracellular matrix secreted by osteoblasts was detected by staining with Alizarin Red S.