Induction of Expression of CD271 and CD34 in Mesenchymal Stromal Cells Cultured as Spheroids

Figure 4

Flow cytometry histograms depicting cells from coculture of ATMSCs and HUVECs in ECM as spheroids and ATMSCs cultured alone in ECM as spheroids. After coculturing ATMSCs and HUVECs in ECM as spheroids, 49.8% of cells were found to express CD271 and 33.1% of cells expressed CD31. When cultured alone in ECM as spheroids, 33.2% of ATMSCs expressed CD271. Histograms drawn in black represent negative controls. Histograms drawn in red represent the expression of the abovementioned molecules by the cell populations. Overlapping curves represent populations of cells that do not express the tested markers. These data are representative of four independent experiments. Mean ± standard deviations of the percentage of cells expressing the surface markers are indicated in parenthesis.