Induction of Expression of CD271 and CD34 in Mesenchymal Stromal Cells Cultured as Spheroids

Figure 7

Immunophenotyping of ATMSCs from spheroids cultured in DMEM/10, MCDB/2, or IF-PCM for 7 days. Cells expressed CD90 but lacked expression of CD11b, CD34, CD79a, CD105, CD106, CD146, and CD271. A minor percentage of ATMSCs from spheroids cultured in MCDB/2 for 7 days also expressed CD271. Histograms drawn in black represent negative controls. Histograms drawn in red represent the expression of the abovementioned molecules by the cell populations. Overlapping curves represent populations of cells that do not express the tested markers. These data are representative of two independent experiments. Mean ± standard deviations of the percentage of cells expressing the surface markers are indicated in parenthesis.