Induction of Expression of CD271 and CD34 in Mesenchymal Stromal Cells Cultured as Spheroids

Table 1

Description of the culture media used in the experiments.

Acronym	Culture medium description	Application
DMEM-LB/2	Low-glucose Dulbecco’s Modified Eagle’s Medium supplemented with 2.2 g/L of sodium bicarbonate (i.e., low bicarbonate), 10 mM HEPES, and 2% of FBS.	Collagenase dilution.
DMEM/10	Low-glucose Dulbecco’s Modified Eagle’s Medium supplemented with 3.7 g/L of sodium bicarbonate, 10 mM HEPES, 1% of penicillin/streptomycin solution, and 10% of FBS.	Culture of ATMSCs (2D and as spheroids). Basal medium for differentiation induction media.
MCDB/2	MCDB 131 supplemented with 2% FBS and 1% of penicillin/streptomycin solution.	Culture of ATMSCs (2D and as spheroids). PCM basal medium.
PCM	Pericyte medium—MCDB 131 supplemented with 2% FBS and 1% of penicillin/streptomycin solution, 2 ng/mL of EGF, 2 ng/mL of FGFb, 2 ng/mL of IGF-1 (LONG®R^3 IGF-I), 5 μg/mL of insulin from bovine pancreas and 1 μg/mL of hydrocortisone.	Culture of ATMSCs (2D and as spheroids).
IF-PCM	IGF-1-free PCM.	Culture of ATMSCs as spheroids.
ECM	Endothelial cell medium—MCDB 131 supplemented with 2% FBS and 1% of penicillin/streptomycin solution, 10 ng/mL of EGF, 5 ng/mL of FGFb, 20 ng/mL of IGF-1, 1 μg/mL ascorbic acid 2-phosphate, 10 IU/mL of heparin and 0.2 μg/mL of hydrocortisone.	Culture of HUVECs (2D) and coculture of ATMSCs with HUVECs as spheroids.