Human Pluripotent Stem Cell Culture: Current Status, Challenges, and Advancement

Table 1

Commonly used commercial media for feeder-free culture of hPSCs.

Medium	Components	Extracellular matrix	XF/CD	Company
mTeSR™1	DMEM/F12, BSA bFGF, TGFβ, insulin, transferrin, cholesterol, lipids, pipecolic acid, GABA, β-mercaptoethanol	Corning® Matrigel®, vitronectin	NA	STEMCELL Technologies
TeSR™2	DMEM/F12, with recombinant HSA, bFGF, TGFβ, insulin, transferrin, cholesterol, lipids, pipecolic acid, GABA, β-mercaptoethanol	Corning® Matrigel®, vitronectin	XF, CD	STEMCELL Technologies
Essential 8™	DMEM/F12 bFGF, TGFβ, insulin, transferrin, selenium, ascorbic acid	Corning® Matrigel®, vitronectin	XF, CD	Thermo Fisher Scientific
TeSR™-E8™	Based on E8 formulation	Corning® Matrigel®, vitronectin	XF, CD	STEMCELL Technologies
StemPro®	DMEM/F12, BSA bFGF, TGFβ, Activin, transferrin, LR3-IGF1, HRG1β	Geltrex®	NA	Thermo Fisher Scientific
PluriSTEM™	DMEM/F12, HSA Activin A, TGFβ1, bFGF, lipids, insulin, transferrin, selenium	Not defined	XF	Millipore

XF: xeno-free; CD: chemically defined medium; NA: not available (neither XF nor CD).