Angiotensin II Attenuates the Bioactivities of Human Endothelial Progenitor Cells via Downregulation of β2-Adrenergic Receptor

Figure 2

ADRB2 agonists stimulate EPC bioactivities. (a) Human EPCs were pretreated with Ang II, and the effects of ADRB2 agonists (isoproterenol and formoterol) were analyzed for test tube formation ability. (b) Migration ability was estimated by a scratch wound healing assay. Each group of EPCs, control cells, or Ang II-treated cells was seeded, followed by treatment with media containing ADRB2 agonists. (c) Quantification of total tube number was performed using ImageJ software. All experiments were performed in triplicates. vs. control, and vs. negative control. (d) Quantification of migrated cells was performed using ImageJ software. Recovery area was analyzed and presented. vs. control; and vs. negative control. (e) Propidium iodide (PI) staining of DNA was detected by flow cytometry of EPCs treated with 100 nM Ang II. (f) Graph of the proportion of cells in the S phase for each group as measured by PI staining. vs. control, and vs. negative control.

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