MicroRNA-1 Regulates the Differentiation of Adipose-Derived Stem Cells into Cardiomyocyte-Like Cells

Figure 2

Identification of ASCs. (a) Cell surface antigen detection results of ASCs using flow cytometry. (A) Negative control ASCs treated with PBS. (B) CD29 FITC-labeled ASCs. The positive rate was 99.8%. (C) CD31PE-A labeled ASCs. The positive rate was 0.3%. (D) CD45 PE-CY5-A labeled ASCs. (b) Osteoblast induction and Alizarin Red staining of ASCs. (A) Control ASCs without induction, dyed with Alizarin Red. Magnification, ×40. (B and C) Differentiated ASCs dyed with Alizarin Red. Red mineralized nodules can be observed. Magnification, ×100 (B) and ×200 (C). (c) Adipogenic induction and Oil Red O staining of ASCs. (A) Control ASCs without induction, dyed with Oil Red O. Magnification, ×40. (B and C) Differentiated ASCs dyed with Oil Red O; cells showed red droplet formation. Magnification, ×100 (B) and ×200 (C).

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