MicroRNA-1 Regulates the Differentiation of Adipose-Derived Stem Cells into Cardiomyocyte-Like Cells

Figure 4

Notch1 and Hes1 protein and gene expression levels in miRNA-1-expressing ASCs after DAPT treatment. ASCs infected with miRNA-1-expressing lentivirus were treated for 7 days with DAPT (Lv-miR-1-DA) or DMSO (Lv-miR-1-DM) or untreated (Lv-miR-1). ASCs infected with control lentivirus were treated for 7 days with DAPT (Lv-NC-DA) or untreated (Lv-NC). Uninfected ASCs were used as negative control. (a) Western blot results of Notch1 and Hes1 protein expression. GAPDH was used as normalization control. (b and c) Quantification of the relative expression level of each protein compared with the control. and as determined by one-way ANOVA. (d and e) Notch1 and Hes1 mRNA levels compared with control. and as determined by one-way ANOVA.

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